AGNOR STAINING PROCEDURE PDF

Fig.3 AgNOR staining with peripheral blood lymphocytes. . The technical aspect of AgNOR staining procedure should be carefully dealt with. Attention should. Conclusions: AgNOR staining can be considered as a useful adjunct to adjunct procedures which can diagnose malignancy at the earliest. Silver staining results are comparable to those obtained. [A standardized AgNOR stain method for formalin fixed and paraffin embedded .. to be a good adjunct to presently available frozen section techniques and cytology.

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AgNOR in cells was counted and size variation and distribution were also recorded. Their size and number reflect nucleolar and cell proliferative activity of tumours Derenzini et al. We conclude that modified AgNOR staining technique is simple, quick and reliable to evaluate cell proliferation by detecting AgNORs size and dispersion.

Two investigators without knowledge of the method of AgNOR stain, tumour type, grade, stage, or disease outcome, performed the Staininv counts, size and distribution. A number of studies carried out in different tumour types demonstrated that malignant cells frequently present a greater AgNOR protein amount than corresponding non-malignant cells. Open in a separate window.

Modified method of AgNOR staining for tissue and interpretation in histopathology

Estimation of AgNORs parameters number, size and distribution has been applied in tumour pathology both for diagnostic and prognostic purposes. Procedurre dots size and dispersion are clearly visible. Typing of AgNOR size and dispersion was also found to be an easy and reproducible alternative to traditional AgNOR counts for differentiating malignant from non-malignant effusions.

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Molecular methods- lab protocols. Rehydration was then performed in several changes of ultrapure distilled water.

After silver-staining, the Procedurd can be easily identified as black dots exclusively localised throughout the nucleolar area, and are called “AgNORs”. Haematoxylin and eosin staining All the sections were stained for Haematoxylin and eosin by two competent histopathologists using the technique by Bukhari et al. In the present study, we adopted both methods and compared their results. We also pay words of praise for all who helped us in performing laboratory works, writing paper, checking statistics and references.

Cytoplasmic granules, organelles and neuroendocrine. Put a few drops of xylene on the preparation and cover with a large No.

AgNOR staining and quantification.

The AgNOR stain Four-micron unstained sections were obtained from the selected areas of tissue to perform the silver staining. In our study, the results of size and dispersion of AgNORs were clearer without the use of counterstain. These parameters should be correlated with the already established but expensive techniques of AgNOR area and size imaging by electron microscopy and flow cytometry, as an economical alternative Khan et al. If black precipitate occurs, slides were not clean or the silver nitrate solution was contaminated.

We compared the results both with and with out counter stain with both tonic agents gold nitrate, sodium thiosulphate Table 1. This article has been cited by other articles in PMC. Cancer Genet Cytogenet 35, Neutral red counter stain was used for few slides to see the contrast, while for others no counter stain was used.

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Patterns of argyrophilic nucleolar organiser regions in pleural and peritoneal effusions. Evaluation of AgNOR in normal brain tissue and astrocytic glioma. These findings are consistent with our results and suggestions that size and configuration of the dots are more important for interpretation of the results in malignant neoplasms.

Banacroft J D, Gamble M, editors. The grading of dots dispersion was performed according to Khan et al. AgNORs can be identified as black dots in the nuclei.

We therefore proposed that mAgNOR would probably be the reflection of the total number of chromosomes or ploidy, and the percentage of cells with five or more AgNOR granules in cells pAgNOR would reflect proliferative activity, as already reported by Mourad et al. In previous methods counter stain Neutral red was used. Over the past 12 years, the “AgNOR method” has been applied in tumour pathology for both diagnostic and prognostic purposes.

Received Aug 12; Accepted Nov Make sure nylon mesh is soaked thoroughly. Journal compilation Blackwell Publishing Ltd. The staining results of our study were brighter with toning agents as consistent with Mourad et al.

Aqueous silver nitrate solution: Cloths and lab benches are normally soiled for life.

Modified method of AgNOR staining for tissue and interpretation in histopathology

Derenzini M, Ploton D. Oil can be removed with xylene. Role of the argyrophilic nucleolar organizer regions in tumor detection and prognosis.